Sa. Patil et al., Detection of disease related immune complexes in the serum of leprosy patients - A novel single step method, J NEUROIMM, 105(1), 2000, pp. 64-68
Mycobacterium leprae antigen and antibody complexes could be detected in th
e serum of leprosy patients using monoclonal antibody ML34 and anti-BCG ant
ibodies by enzyme-linked immunosorbent assay. This simplified system detect
s disease related complexes without the need for isolating and purifying th
em from the serum. Immune complexes captured using monoclonal antibody ML34
revealed positivity in seven out of eight neuritic, two out of nine tuberc
uloid (TT), five out of ten borderline tuberculoid (BT), four out of ten bo
rderline lepromatous (BL) and four out of ten lepromatous (LL), leprosy cas
es. One of the controls also showed immune complex of an IgM type. Anti-BCG
based IgG immune complexes assay revealed positivity in six out of eight n
euritic, one out of nine TT, four out of ten BT, two out of ten BL, four ou
t of ten LL leprosy cases, and two out of 24 healthy controls. IgM type of
mycobacterial immune complexes were almost negligible. Capture of complexes
using monoclonal antibody ML34 which is against lipoarabinomannan of M. le
prae seems to work better than polyclonal anti-BCG antibody. The probable r
ole of immune complexes in nerve damage needs to be evaluated, as very high
levels of immune complexes are found in neuritic leprosy by both the assay
s. The above test would be useful in immunodiagnosis of neuritic leprosy an
d also in cases where antibody response is not detectable because of the fo
rmation of immune complexes. (C) 2000 Published by Elsevier Science B.V. Al
l rights reserved.