Interleukin-1 beta induction of the chemokine RANTES promoter in the humanastrocytoma line CH235 requires both constitutive and inducible transcription factors

The chemokine RANTES is an important mediator of inflammatory processes. In this report, we describe the DNA sequence and transcription factor require ments for interleukin-1 beta (IL-1 beta) induction of the RANTES promoter i n the human astrocytoma line CH235. RANTES promoter sequences between -278 and +55 are sufficient for IL-1 beta-inducibility. In vitro DNA binding ass ays demonstrate constitutive binding of Sp1, HMG, Ets domain, and bZIP fami ly members to their cognate sites in the RANTES promoter, whereas NF-kappa B and IRF-1 bind in an IL-1 beta-inducible manner. IL-1 beta-inducibility o f the RANTES promoter requires both constitutive and inducible transcriptio n factors. The formation of a higher order nucleoprotein complex, or 'enhan ceosome', may be critical for IL-1 beta induction of the RANTES promoter. ( C) 2000 Elsevier Science B.V. All rights reserved.