In vivo glucose detection by homonuclear spectral editing

A frequency-selective multiple-quantum-coherence spectral editing pulse seq uence, Ssel-MQC, was implemented for the detection of the beta H1-glucose r esonance at 4.63 ppm in rat brain in vivo. Unwanted signal suppression and glucose coherence transfer pathway selection were performed with magnetic f ield gradients. To optimize sensitivity, the sequence was executed with sur face coil signal reception and adiabatic RF pulse transmission. The glucose editing capabilities of Ssel-MQC were first evaluated in vitro. Ssel-MQC a chieved excellent water suppression (suppression factor >10(5)), at the exp ense of an similar to 60% loss of the glucose signal due to incomplete cohe rence transfer pathway selection. Next, the sequence was used for in vivo g lucose detection in normal rat brain during D-glucose infusion and in the b rain of diabetic rats prior to and following insulin infusion. Magn Reson M ed 43:621-626, 2000. (C) 2000 Wiley-Liss, Inc.