Interleukin 2 induction of proliferation in resting T lymphocytes requirescontact with monocytes

Citation
S. Pesoa et al., Interleukin 2 induction of proliferation in resting T lymphocytes requirescontact with monocytes, MEDICINA, 60(2), 2000, pp. 202-210
Citations number
34
Categorie Soggetti
Medical Research General Topics
Journal title
MEDICINA-BUENOS AIRES
ISSN journal
00257680 → ACNP
Volume
60
Issue
2
Year of publication
2000
Pages
202 - 210
Database
ISI
SICI code
0025-7680(2000)60:2<202:I2IOPI>2.0.ZU;2-0
Abstract
Resting human T cells are known to express significant numbers of intermedi ate but none or barely detectable low and high affinity IL-2 receptors (IL- 2R). IL-2 alone failed to induce proliferation In these cells. However, in presence of small proportion of autologous monocytes, as low as 22 pM, IL-2 induced high levels of proliferation in resting T cells. Introduction of a semi permeable membrane between the two cell types or addition of an anti- CD11b mAb inhibited such induction of proliferation by IL-2. Neither recomb inant IL-1 nor IL-1-containing cell-free extracts from activated monocytes substituted for intact monocytes. Autologous B cells failed to replace mono cytes. Using antigen-specific cloned human T cells we have shown a lack of requirement for antigen. The proliferation was inhibited by anti-IL-2R alph a mAb. IL-2 appears to be unique since neither IL-4 nor IL-6, alone or in p resence of monocytes, led to induction of proliferation in resting T cells. Combination of IL-2 and monocytes induced proliferation in all T cell subp opulations (CD4(+), CD8(+), CD45RA(+) and CD45RO(+)) and antigen-specific c lones examined. It also induces mRNA and surface expression of IL-2R alpha, appearance of high affinity IL-2R and induction of proliferation in large proportions of T cells. As in humans, the IL-2 induction of proliferation i n murine resting T cells required contact with syngeneic monocytes, suggest ing that such a mechanism of T cells activation is highly conserved.