Detection of intracytoplasmic cytokines by flow cytometry

Citation
Ma. Santiago et al., Detection of intracytoplasmic cytokines by flow cytometry, MEM I OSW C, 95(3), 2000, pp. 401-402
Citations number
16
Categorie Soggetti
Medical Research General Topics
Journal title
MEMORIAS DO INSTITUTO OSWALDO CRUZ
ISSN journal
00740276 → ACNP
Volume
95
Issue
3
Year of publication
2000
Pages
401 - 402
Database
ISI
SICI code
0074-0276(200005/06)95:3<401:DOICBF>2.0.ZU;2-7
Abstract
Flow cytometry has been used as a powerful technique for studying cell surf ace antigen expression as well as intracellular molecules. Its capability o f analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous populations. In this respect, several techniques have been developed durin g the past few yeats to study cytokine-producing cells by flow cytometry in humans and several animal models.