Detection of intracytoplasmic cytokines by flow cytometry

Flow cytometry has been used as a powerful technique for studying cell surf ace antigen expression as well as intracellular molecules. Its capability o f analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous populations. In this respect, several techniques have been developed durin g the past few yeats to study cytokine-producing cells by flow cytometry in humans and several animal models.