The influence of carbon sources and mononucleotides on the production of extracellular alkaline phosphatase by Bacillus intermedius

The biosynthesis of extracellular alkaline phosphatase in the streptomycin- resistant strains Bacillus intermedius S3-19 and S7 in the presence in the medium of 5'-nucleoside monophosphates and different sources of carbon-gluc ose, sodium pyruvate, sodium lactate, or glycerol-was studied. It was estab lished that, in the presence of mononucleotides, the content of extracellul ar alkaline phosphatase in both strains increased; the maximal effect was c aused by 5'-AMP at a concentration of 20 mu g/ml. In medium with a low orth ophosphate content, where active biosynthesis of alkaline phosphatase occur red, 1% glucose and 0.5% pyruvate stimulated this process 2.5-4 times, and 2% sodium lactate and sodium pyruvate, on the contrary, inhibited it by 20- 40%. Analysis of the dynamics of growth and accumulation of extracellular p hosphatase in the presence of different sources of carbon in the medium giv es evidence of an interrelationship between the biosynthesis of alkaline ph osphatase and carbon metabolism in Bacillus intermedius.