Extracellular factors affecting the adhesion of Pseudomonas fluorescens cells to glass surfaces

Two factors affecting the adhesion of Pseudomonas fluorescens to glass surf aces were revealed in the culture liquid (CL) of this bacterium. One of the se factors, adhesin, which is responsible for cell adhesion, was found to b e a protein substance located both at the cell surface and in the CL, Bacte rial cells grown in rich LB medium were less adhesive than cells grown in m inimal M9 medium 1. The adhesive capacity of cells was independent of the g rowth phase. The other factor, antiadhesin (AA), which reduces cell adhesio n, was found only in the CL. AA concentration in the CL increased with the culture age.