Citation
Ey. Kotova et al., Effects of various fluorochromes and competition between labeled oligonucleotides on their hybridization to oligonucleotides immobilized on biological microchips, MOL BIOL, 34(2), 2000, pp. 207-214
Citations number
10
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY
ISSN journal
00268933
→ ACNP
Volume
34
Issue
2
Year of publication
2000
Pages
207 - 214
Database
ISI
SICI code
0026-8933(200003/04)34:2<207:EOVFAC>2.0.ZU;2-F
Abstract
Some technical tips are described on how to improve the discrimination betw
een perfect and imperfect duplexes formed by hybridization of fluorescently
labeled oligonucleotides to biological microchips. Model experiments were
performed to assess the precision of the method. Effects of labeling on the
efficiency of hybridization and some properties of competitive hybridizati
on were studied using short synthetic oligonucleotides and three most popul
ar fluorochromes as examples.