Cloning and characterization of bonnet monkey GnRH receptor

Gonadotrophin releasing hormone (GnRH) plays an important role in the repro ductive processes of both males and females. It is synthesized by the hypot halamus and binds to a specific receptor on the pituitary to bring about th e release of the gonadotrophins, lutineizing hormone and follicle stimulati ng hormone. which in turn bring about the release of the gonadal steroids. Although the structure of the GnRH receptor (GnRHR) has been elucidated fro m a number of sources, no information is available about the receptor from the non-human primate species. Here we report the cloning and characterizat ion of the receptor from the pituitary of the bonnet monkey. Antiserum to a bacterially expressed recombinant fragment was used in Western blot analys is and fluorescence microscopy to demonstrate the presence of GnRHR in both human and monkey placentae and pituitary.