Proteinchip((R)) surface enhanced laser desorption/ionization (SELDI) massspectrometry: a novel protein biochip technology for detection of prostatecancer biomarkers in complex protein mixtures
Gl. Wright et al., Proteinchip((R)) surface enhanced laser desorption/ionization (SELDI) massspectrometry: a novel protein biochip technology for detection of prostatecancer biomarkers in complex protein mixtures, PROSTATE C, 2(5-6), 1999, pp. 264-276
Improving early detection, diagnosis, treatment monitoring and prognosis of
cancer will require rapid and high throughput detection, identification, a
nd measurement of multiple biomarkers. In this study, we demonstrate the ve
rsatility of the innovative SELDI ProteinChip(R) MS technology for the rapi
d, reproducible and simultaneous identification of four well-characterized
prostate cancer-associated (PCA) biomarkers, prostate specific antigen (fre
e and complexed forms), prostate specific peptide, prostate acid phophatase
and prostate specific membrane antigen in cell lysates, serum and seminal
plasma. Proteins corresponding to the mass of these biomarkers could readil
y be captured and detected using either chemically defined or antibody coat
ed ProteinChip(R) arrays. Several (yet to be identified) proteins were foun
d upregulated in cell lysates of pure populations of PCA cells procured by
laser capture microdissection (LCM) when compared with mass spectra of norm
al cell lysates. Coupling LCM with SELDI provides tremendous opportunities
to discover and identify the signature proteins associated with each stage
of tumor development. Collectively, these observations demonstrate the pote
ntial of SELDI for the discovery and simultaneous detection of and clinical
assay development for PCA biomarkers in complex biological mixtures.