H. Schneider et al., Patterns of Shaker family gene expression in single identified neurons of the American lobster, Homarus americanus, RECEPT CHAN, 7(1), 2000, pp. 53-64
The patterns of expression of voltage gated potassium channel genes of the
Shaker family have been mapped in identified neurons of the lobster (Homaru
s americanus) ventral nerve cord using a single cell reverse transcriptase
polymerase chain reaction procedure. Using specific oligonucleotides derive
d from the sequences of the shakerm, shab, and shaw genes of the spiny lobs
ter, Panulirus interruptus, we detected the corresponding potassium channel
DNA fragments from Homarus americanus. The Homarus DNA fragments are 87-98
% identical at the nucleotide level to the Panulirus DNA fragments. We used
the Panulirus primers to measure the complement of RNAs for shaker, shab,
and shan: in single identified cells that use GABA, glutamate, octopamine o
r serotonin as chemical messengers. Shaker and shaw RNAs were found in all
four identified neuron types but shab RNA was not detected in serotonin cel
ls under the present experimental conditions. All cells expressed alpha-tub
ulin RNA, which serves as an internal control suggesting that cells are int
act after dissection. In glial cells that surround the neuronal cell bodies
, the potassium channel genes are expressed at low to non-detectable levels
.