Use of weak organic protolytes to form a pH gradient during chromatofocusing

Citation
Ab. Tessman et al., Use of weak organic protolytes to form a pH gradient during chromatofocusing, RUSS J PH C, 73(8), 1999, pp. 1320-1323
Citations number
11
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY
ISSN journal
00360244 → ACNP
Volume
73
Issue
8
Year of publication
1999
Pages
1320 - 1323
Database
ISI
SICI code
0036-0244(199908)73:8<1320:UOWOPT>2.0.ZU;2-7
Abstract
Internal pH gradients for sorbents with grafted oligoethyleneamines were ob tained by using single-component eluents containing weak protolytes. The sh apes of the experimental pH profiles were explained. The experimental and c alculated pH profiles were compared. An approach to selecting the sorbent, initial solution, and eluent in order to obtain a desired elution profile w as proposed.