Two-dimensional TLC separation and mass spectrometric identification of anthraquinones isolated from the fungus Dermocybe sanguinea

A new two-dimensional TLC technique was developed to separate substituted a nthraquinones on silica prates using n-pentanol-pyridine-methanol (6:4:3, v /v/v) and toluene-ethyl acetate-ethanol-formic acid (10:8:1:2, v/v/v/v) as eluents. The good separation power of the new technique was demonstrated by applying it to the analysis of complex anthraquinone mixtures isolated fro m the Scandinavian Dermocybe sanguinea. Emodin, physcion, endocrocin, dermo lutein, dermorubin, 5-chlorodermorubin, emodin-1-beta-D-glucopyranoside, de rmocybin-1-beta-D-glucopyranoside and dermocybin, and five new, earlier in D. sanguinea unidentified compounds, 7-chloroemodin, 5,7-dichloroemodin, 5, 7-dichloroendocrocin, 4-hydroxyaustrocorticone and austrocorticone, were se parated and identified on the basis of Ri values, UV/Vis spectra and mass s pectra.