Indirect neurotrophic effect of neuropeptide ZNC(C)PR on PC12 cells via peptide-stimulation of C6 cells

AIM: To understand the mechanism of neurotrophic action of neuropeptide ZNC (C)PR and its effect on which could affect both growth and apoptosis of CG cells. METHODS: Effects of ZNC(C)PR-treated C6 conditioned medium was obser ved on on culture of PC12 cells. The development of PC12 cells was determin ed by ratio of neurite-bearing cells in the total cells. The specific bindi ng of ZNC(C)PR on CG cells was determined by radioligand binding assay (RBA ). RESULTS: ZNC(C) PR-treated C6 conditioned medium increased the ratio of neurite-bearing PC12 cells by 36 % compared to the untreated C6 conditioned medium or to a mixture of ZNC (C)PR with the untreated C6 conditioned medi um. RBA showed a specific binding site of ZNC(C)PR on CG cells with K-d val ue of 2.74 nmol.L-1 and B-max value of 19 pmol.g(-1) protein. CONCLUSION: Z NC(C)PR enhanced C6 cells induced secretion of some neurotrophic factors wh ich acted as enhancers for PC12 cells differentiation, through its specific receptor sites on the neuroglioma cell.