Effect of lipanthyl on mRNA expression of endothelin-1 and nitric-oxide synthase in atherosclerotic vessel wall in rabbits

AIM: To study the mechanism of regression of atherosclerosis (AS) by lipant hyl. METHODS: Experimental atherosclerotic rabbits created by damaging the abdominal aortic endothelium and feeding with high fat diet for 8 wk were t hen treated with lipanthyl 15 mg kg(-1).d(-1) for 16 wk. Expression of endo thelin (ET)-1 mRNA and nitric oxide synthase (NOS) mRNA in atherosclerotic vessel wall was measured by in situ hybridization and reverse transcription polymerase chain reaction (RT-PCR), respectively. RESULTS: After lipanthyl administration for 16 wk, ET-1 mRNA expression was reduced, and integral o ptical density (IOD) and area of hybridization granule were observed to be (49 113 +/- 16 868) and (2448 +/- 621) mu m(2) in lipanthyl group and (65 1 88 +/- 10 113) and (3028 +/- 352) mu m(2) in atherosclerotic group, respect ively. Regarding inducible NOS mRNA expression, IOD and area were decreased by 25.5 % and 53.3 %, respectively, whereas endothelial NOS mRNA expressio n was increased. CONCLUSION: Restoration of the disturbed ET-1 mRNA/NOS mRN A balance by lipanthyl might be one of its mechanisms leading to regression of atherosclerosis.