A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus

Authors
Eroglu, C Yildiz, E Ozturk, M Pinarbasi, E
Citation
C. Eroglu et al., A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus, ACT VIROLOG, 44(1), 2000, pp. 29-33
Citations number
23
Categorie Soggetti
Microbiology
Journal title
ACTA VIROLOGICA
ISSN journal
0001723X → ACNP
Volume
44
Issue
1
Year of publication
2000
Pages
29 - 33
Database
ISI
SICI code
0001-723X(200002)44:1<29:AHSASE>2.0.ZU;2-C
Abstract
In this study, a 178 amino acids long portion of the hepatitis C virus (HCV ) core gene was cloned sequenced expressed in Escherichia coli, and purifie d. The resulting antigen (C178) was tested with human sera enzyme-linked im munosorbent assay (ELISA) in order to assess its ability to diagnose HCV. I t was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3(rd) generation enzyme immunoassay (ETA) as HCV-positive, had antibodie s against the C178 antigen. This antigen gave no false positive results whe n tested with anti-HCV-negative sera.