C. Eroglu et al., A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus, ACT VIROLOG, 44(1), 2000, pp. 29-33
In this study, a 178 amino acids long portion of the hepatitis C virus (HCV
) core gene was cloned sequenced expressed in Escherichia coli, and purifie
d. The resulting antigen (C178) was tested with human sera enzyme-linked im
munosorbent assay (ELISA) in order to assess its ability to diagnose HCV. I
t was shown by ELISA that 92% of the patients sera, diagnosed previously by
a 3(rd) generation enzyme immunoassay (ETA) as HCV-positive, had antibodie
s against the C178 antigen. This antigen gave no false positive results whe
n tested with anti-HCV-negative sera.