Detection of small round structured viruses in stool specimens from outbreaks of gastroenteritis by electron microscopy and reverse transcription-polymerase chain reaction

Authors
Otsu, R Ishikawa, A Mukae, K
Citation
R. Otsu et al., Detection of small round structured viruses in stool specimens from outbreaks of gastroenteritis by electron microscopy and reverse transcription-polymerase chain reaction, ACT VIROLOG, 44(1), 2000, pp. 53-55
Citations number
12
Categorie Soggetti
Microbiology
Journal title
ACTA VIROLOGICA
ISSN journal
0001723X → ACNP
Volume
44
Issue
1
Year of publication
2000
Pages
53 - 55
Database
ISI
SICI code
0001-723X(200002)44:1<53:DOSRSV>2.0.ZU;2-V
Abstract
In testing 60 stool specimens small round structured virus (SRSV) particles were detected in 35 (58%) specimens from all 17 outbreaks of gastroenterit is by electron microscopy (EM), while SRSV genes were found in 36 (60%) spe cimens from 15 outbreaks by reverse transcription-polymerase chain reaction (RT-PCR) by use of 2 primer pairs. Specimens from 2 outbreaks were found S RSV particles-positive by EM but SRSV genes-negative by RT-PCR. EM remains the basic examination method for diagnosis of SRSV agents.