Kinetic determination of acid phosphatase activity by double injection flow analysis with electrochemical detection

A two-points kinetic procedure has led to an enzyme assay for the measureme nt of total and prostatic acid phosphatase (PAP) activity by flow injection analysis with electrochemical detection. A double injection valve is emplo yed for the simultaneous injection of the substrate (alpha-naphthyl phospha te) and enzyme. Successive injections are directed into different reaction coils for amperometric measurement of the alpha-naphthol produced at differ ent times. Total and prostatic activity is determined in control sera and r esults were compared with those obtained spectrophotometrically. (C) 2000 E lsevier Science B.V. All rights reserved.