Diversification and selection mechanisms for the production of protein repertoires - Lessons from the immune system

Authors
Neuberger, MS Sale, JE Cumbers, SJ Jolly, CJ Bemark, MP Ehrenstein, MR Lanoue, A Bruggemann, M Batista, FD Davies, SL Williams, GT
Citation
Ms. Neuberger et al., Diversification and selection mechanisms for the production of protein repertoires - Lessons from the immune system, APPL BIOC B, 83(1-3), 2000, pp. 53-60
Citations number
15
Categorie Soggetti
Biotecnology & Applied Microbiology","Biochemistry & Biophysics
Journal title
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN journal
02732289 → ACNP
Volume
83
Issue
1-3
Year of publication
2000
Pages
53 - 60
Database
ISI
SICI code
0273-2289(200001/03)83:1-3<53:DASMFT>2.0.ZU;2-4
Abstract
The physiological mechanism for producing antigen-specific antibodies is ba sed on a two-phase neo-Darwinian process: the first phase consists of diver sity generation (formation of the repertoire), and the second phase is anti gen-mediated selection. In this article, we consider how the natural immuno globulin gene-diversification processes can be exploited both in vivo and i n vitro in order to allow the generation of novel antibody (and heterologou s protein) repertoires.