A METHOD FOR THE LARGE-SCALE ISOLATION OF HIGH TRANSFORMATION EFFICIENCY FUNGAL GENOMIC DNA

Authors
ZHANG DL YANG YF CASTLEBURY LA CERNIGLIA CE
Citation
Dl. Zhang et al., A METHOD FOR THE LARGE-SCALE ISOLATION OF HIGH TRANSFORMATION EFFICIENCY FUNGAL GENOMIC DNA, FEMS microbiology letters, 145(2), 1996, pp. 261-265
Citations number
15
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
145
Issue
2
Year of publication
1996
Pages
261 - 265
Database
ISI
SICI code
0378-1097(1996)145:2<261:AMFTLI>2.0.ZU;2-Z
Abstract
A procedure for isolation of genomic DNA from the zygomycete Cunningha mella elegans and other filamentous fungi and yeasts is reported. This procedure involves disruption of cells by grinding using dry ice, rem oval of polysaccharides using cetyltrimethylammonium bromide and by ph enol extractions, and precipitation of DNA with isopropanol at room te mperature. The isolation method produced large scare (approximate 1 mg DNA/5 g wet cells) and highly purified high molecular mass DNA. Sau3A I partially digested DNA showed high transformation efficiency (> 10(6 )/100 ng DNA) when ligated to ZAP-express lambda vector.