CENTROMERIC AND NONCENTROMERIC ADE2-SELECTABLE FRAGMENTATION VECTORS FOR YEAST ARTIFICIAL CHROMOSOMES IN AB1380

Authors
HEUS JJ DEWINTHER MPJ VANDEVOSSE E VANOMMEN GJB DENDUNNEN JT
Citation
Jj. Heus et al., CENTROMERIC AND NONCENTROMERIC ADE2-SELECTABLE FRAGMENTATION VECTORS FOR YEAST ARTIFICIAL CHROMOSOMES IN AB1380, PCR methods and applications, 7(6), 1997, pp. 657-660
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
PCR methods and applications → ACNP
ISSN journal
10549803
Volume
7
Issue
6
Year of publication
1997
Pages
657 - 660
Database
ISI
SICI code
1054-9803(1997)7:6<657:CANAFV>2.0.ZU;2-9
Abstract
We have constructed a set of fragmentation vectors for the truncation of either the centromeric or the noncentromeric end of YACs containing a human DNA insert. These vectors carry ADE2 or HIS5 as the selectabl e marker, enabling direct use in AB1380, the host strain of most publi cly available YAC libraries. Centromeric fragmentation vectors for AB1 380 have not been reported previously; the noncentromeric vectors show high frequencies of fragmentation.