Use of anion exchange resin-packed capillary column for rapid detection ofanti-double-stranded DNA antibody in systemic lupus erythematosus serum

An anti-double-stranded (ds) DNA antibodies in serum-detection system was d eveloped based on flow immunoassay with packed-capillary column. Alkaline p hosphatase conjugated DNA (ALP-DNA) and anti-dsDNA antibody were separated from unreacted ALP-DNA on the basis of the difference in isoelectric point. A linear anti-dsDNA antibody dose-response curve was obtained between lumi nescence intensity and concentration of anti-dsDNA antibody in the range 25 -200 IU/mL. This simple technique permits the assay of anti-dsDNA autoimmun e antibodies within 7 minutes. These results give reduced detection times c ompared with those previously reported (Lim et al., 1999) through the use o f capillary columns. (C) 2000 John Wiley & Sons, Inc.