Biological characteristics of golden ide reovirus (GIRV)

The reovirus described in this study was demonstrated by infection trials t o be an "orphan" virus not known to cause a disease. No mortality or other discernible signs of a disease could be initiated, but viral infections wer e established at a high rate in golden ide (up to 100%) and to a lower degr ee in carp (up to 40%). Virus replication could not be induced in rainbow t rout. In vitro investigations revealed a considerable enhancement of infect ivity when the foetal bovine serum in the virus growth medium was replaced with trypsin. Temperatures between 15 degrees C and 20 degrees C proved to be optimal for virus replication. Besides growth in EPC and FHM cells the v irus replicated in CHSE and BF-2 cells and to a very low degree in RTG-2 ce lls.