We have used the rat tyrosine hydroxylase promotor to overexpress MYCN in t
he neural crest of transgenic mice, resulting in a mouse model for neurobla
stoma. Using PCR analysis of microsatellite markers, we conducted a genome-
wide analysis in tumors from these animals. Regions of chromosomes 1, 3, 10
, 11, 14, and 18 were affected in 20-50% of tumors. Analysis of a subset of
these tumors by comparative genomic hybridization was consistent with the
microsatellite data. The changes on mouse chromosomes 1, 11, 14, and 18 wer
e syntenic with corresponding: regions of loss of heterozygosity in human n
euroblastoma, suggesting that genes implicated in the mouse tumors may also
play a role in the pathogenesis of the human disease. One-third of the mou
se tumors shared abnormalities on chromosomes 1, 3, and 10, whereas the rem
ainder of tumors did not show this combination. These data suggest that gen
etic mutations on chromosomes 1, 3, and 10 cooperate in the pathogenesis of
neuroblastoma and that neuroblastoma in the mouse arises from at least two
distinct genetic pathways, one of which is dependent on lesions in chromos
omes 1, 3, and 10, the other of which is not.