O. Itoh et al., Sensitive ESR determination of intracellular oxidative stress by using acyl-protected hydroxylamines as new spin reagents, CHEM LETT, (4), 2000, pp. 304-305
Several acyl-protected hydroxylamines were synthesized as new spin reagents
for ESR measurements of intracellular oxidative stress. These compounds we
re stable non-radical compounds, but were easily deprotected with esterase
to yield hydroxylamines, which were oxidized by oxidants to yield ESR- dete
ctable nitroxide radical. Using an acyl-protected hydroxylamine, a highly s
ensitive ESR determination procedure was successfully conducted to analyze
oxidative stress in human leukocytes.