This payer demonstrates the possibilities in transfering HPLC methods from
porous to non-porous stationary phases. The procedures are transferred from
125 or 250 mm columns packed with porous stationary phases to 33 mm column
s packed with non-porous particles. It is demonstrated that fast HPLC using
non-porous columns reduces analysis times by a factor four to eight. Preci
sion is comparable to HPLC with porous stationary phases. The costs for HPL
C with porous and with nonporous packing materials are similar. The impleme
ntation of those fast HPLC columns is easy because standard equipment can b
e used.