Fast HPLC with non-porous packing materials in the pharmaceutical industry

This payer demonstrates the possibilities in transfering HPLC methods from porous to non-porous stationary phases. The procedures are transferred from 125 or 250 mm columns packed with porous stationary phases to 33 mm column s packed with non-porous particles. It is demonstrated that fast HPLC using non-porous columns reduces analysis times by a factor four to eight. Preci sion is comparable to HPLC with porous stationary phases. The costs for HPL C with porous and with nonporous packing materials are similar. The impleme ntation of those fast HPLC columns is easy because standard equipment can b e used.