Mp. Fournet-bourguignon et al., Phenotypic and functional changes in regenerated porcine coronary endothelial cells - Increased uptake of modified LDL and reduced production of NO, CIRCUL RES, 86(8), 2000, pp. 854-861
Porcine coronary arteries with regenerated endothelium exhibit impaired end
othelium-dependent relaxations, Experiments were designed to analyze the st
ructural and functional changes occurring in regenerated endothelial cells.
Primary cultures from regenerated endothelium contained giant endothelial
cells, with an increased number of cells with diameter >14.5 mu m, a reduce
d ability to proliferate, and signs of apoptosis, The uptake of fluorescent
acetylated LDL was increased 2-fold in cultures from regenerated endotheli
um. The increased uptake of acetylated LDL was confirmed ex vivo in injured
coronary arteries. In cultures from regenerated endothelium, cGMP producti
on was decreased under basal conditions and during stimulation with seroton
in, bradykinin, and A23187. Thus, during regeneration, there is accelerated
senescence of endothelial cells accompanied by increased incorporation of
modified LDL and reduction of NO production without decrease in endothelial
NO synthase expression. These alterations help to explain the altered endo
thelium-dependent responses 28 days after balloon injury.