Diagnosis of Enterocytozoon bieneusi by PCR in stool samples eluted from filter paper disks

We report a PCR-based assay for the detection of Enterocytozoon bieneusi. W e extracted DNA from feces which had been applied to filter paper disks and evaluated four preserving solutions. Infected specimens were identified by electrophoresis of amplicons from concentrated formalin-fixed samples and unconcentrated fresh feces. Our findings demonstrate that this methodology is effective for sample collection, mailing, and diagnosis of this pathogen .