Copper-dependence of mitochondrial DNA rearrangements in Podospora anserina

Rearrangements of the mitochondrial DNA (mtDNA) are a hallmark of senescenc e in wild-type strains of the ascomycete P, anserina. These rearrangements include the systematic amplification of the first intron (pl-intron) of the cytochrome oxidase subunit-I gene (CoI) as a circular DNA molecule (p1DNA) . In addition. deletions and amplifications of other regions of the mtDNA o ccur. The molecular basis of the underlying processes is not understood in detail. A comparative analysis of the wild-type strain and of the long-live d mutant grisea, affected in the uptake of copper, revealed that mtDNA inst abilities are dependent on the availability of cellular copper. In the muta nt, the first steps in the corresponding pathway, including the transcripti on of the Col gene, the splicing of the pl-intron and the transposition of this mobile element, are not impaired. In contrast. recombination processes between short direct repeats, as well as rearrangements between two tandem intron copies leading to the formation of p1DNA, appear to be affected. Ad ditional copper in the growth medium rescues this molecular phenotype. We s uggest that copper is a cofactor of a component of the molecular machinery leading to the characteristic age-related mtDNA rearrangements.