Polymorphism in the porcine NGFB gene detected by DGGE and its linkage mapping

The porcine nerve growth factor beta gene (NGFB) belongs to a conserved syn tenic group on chromosome 4. The objective of this study was to Find a simp ly detectable polymorphism and use it in genetic studies. The new set of PC R primers was used to amplify a 448 bp Fragment of the gene. Polymorphism w as studied by denaturing gradient gel electrophoresis (DGGE). One hundred a nd fifty-four pigs belonging to eight breeds were studied and two alleles, A and B, were detected. Frequencies of B allele were: Large White - 0.50, L andrace - 0.96, Pietrain - 0.81 and Black Pied Prestice - 0.83. Czech Meat Pig (14 pigs), Hampshire (6), Duroc (2) and Meishan (8) were monomorphic fo r allele B. Data from three Hohenheim PiGMaP F-2 pedigrees were used for li nkage mapping. The sex average locations of the NGFB gene were tin Kosambi cM) 101.8 (Wild Boar x Pietrain), 95.5 (Meishan x Pietrain) and 89.5 (Wild Boar x Meishan), respectively.