The purposes of this study were to determine whether microorganisms can be
isolated from the membranes of stethoscopes used by clinicians and nurses,
and to analyse whether or not the degree of bacterial colonization could be
reduced with different cleaning methods. We designed a transversal before-
after study in which 122 stethoscopes were examined. Coagulase negative sta
phylococci (which are also potentially pathogenic microorganisms) were isol
ated together with 13 other potentially pathogenic microorganisms, includin
g S. aureus, Acinetobacter sp. and Enterobacter agglomerans. The most effec
tive antiseptic was propyl alcohol. Analysis of the cleaning habits of the
Emergency Department (ED) staff, showed that 45 % cleaned the stethoscope a
nnually or never. The isolation of potentially pathogenic microorganisms su
ggests that the stethoscope must be considered as a potential vector of inf
ection not only in the ED but also in other hospital wards and out-patient
clinics.