THE INFLUENCE OF GENOTYPE AND MEDIUM ON RYE (SECALE-CEREALE L) ANTHERCULTURE

Anthers of three rye inbred lines - L9, L318, Dw28, one synthetic hybr id - F-1(5) and one variety, Darikowskie Zlote (DZ), were cultured on two media based on N6, and two based on P2 components. The induction r ate significantly depended on genotype - the best results were obtaine d for line L318 and the lowest percentage of responding anthers was no ticed in line L9. There was no universal medium for all tested genotyp es. The highest induction rate (IR) for lines L318, L9 and hybrid F-1( 5) was obtained on medium CI (11.94, 0.71 and 1.75% respectively). For DZ, the P2I medium was better than the others while for Dw28 CIP turn ed to be as suitable as CI. A highly significant interaction between g enotype and medium was proved. Single anthers of DZ, L318 and L9 produ ced embryos on CI, CIP and P2I media. They did not develop into plants but after transferring them to CS1.7 medium, a secondary, embryogenic callus was obtained. Such a reaction has not been described in rye un til now. In spite of a relatively high IR, plant regeneration was rath er poor. An elaboration of this step in haploid production is needed. Most of the analyzed calluses and microspore derived regenerants prove d to be haploids, according to flow cytometry analysis. Plants treated with colchicine were doubled haploids.