R. Nikolic et al., EVALUATION OF AGRONOMIC TRAITS IN TISSUE-CULTURE - DERIVED PROGENY OFBIRDS-FOOT TREFOIL, Plant cell, tissue and organ culture, 48(1), 1997, pp. 67-69
Two newly selected Lotus corniculatus cultivars (Bokor and Zora), intr
oduced recently as pasture legumes, were studied with respect to their
in vitro regeneration capacity and field performance of R-1 regenerat
es. Multiple buds were induced in callus tissues derived from apical b
uds, petioles, leaf and cotyledon explants. The rooted regenerates, ac
climated in a greenhouse, were fertile. Seeds from the R-1 generation
were sown in the field and the plants found to be superior to controls
in stem height and green mass production.