K. Yamamoto et al., Negative regulation of the bolA1p of Escherichia coli K-12 by the transcription factor OmpR for osmolarity response genes, FEMS MICROB, 186(2), 2000, pp. 257-262
To test whether OmpR is involved in regulation of the bolAlp, we investigat
ed possible effects of ompR mutation on transcription from bolAlp. In vivo,
bolAlp was found to be repressed by OmpR. Furthermore in vitro, the phosph
o-OmpR was found to bind to the OmpR binding region of bolAlp and repress t
he transcription by E sigma(S) or E sigma(D). These results suggest that th
e phosphorylated form of OmpR is a negative regulator for the transcription
of the bolAlp promoter. (C) 2000 Federation of European Microbiological So
cieties. Published by Elsevier Science B.V. All rights reserved.