F. Devreker et al., Noninvasive assessment of glucose and pyruvate uptake by human embryos after intracytoplasmic sperm injection and during the formation of pronuclei, FERT STERIL, 73(5), 2000, pp. 947-954
Objective: To improve in vitro culture conditions and human embryo selectio
n before transfer after IVF with intracytoplasmic sperm injection (ICSI).
Design: A controlled, randomized, prospective study.
Setting: University hospital-based IVF-ET program.
Patient(s): Couples undergoing ICSI.
Intervention(s): Culture of human embryos in the presence of 1 mM or 5.56 m
M glucose and metabolic measurements with the use of noninvasive microfluor
escence assays immediately after ICSI to the time of transfer.
Main Outcome Measure(s): Embryo development, implantation rate, and glucose
and pyruvate uptake.
Result(s): Fertilization rates, early embryo development, and implantation
rates were not significantly different between 1 mM and 5.56 mM glucose. Py
ruvate uptake was significantly higher during the formation of the pronucle
i, at 15 +/- 0.7 and 11.4 +/- 1.3 pmol/embryo/h for fertilized and unfertil
ized oocytes, respectively. Pyruvate uptake did not correlate with cleavage
stage or embryo morphology. However, during the second day of incubation,
pyruvate uptake was significantly higher for the untransferred embryos of p
regnant women compared with nonpregnant women, at 17.9 +/- 1.5 and 10.8 +/-
1.0 pmol/embryo/h, respectively.
Conclusion(s): The increased level of pyruvate uptake during fertilization
reflects the increased demand for energy necessary for the formation of the
pronuclei. However, the metabolic measurements could not improve the selec
tion of embryos with the best implantation potential. Finally, the reductio
n of glucose concentration in the culture medium failed to improve embryo v
iability. (Fertil Steril(R) 2000;73:947-54. (C) 2000 by American Society fo
r Reproductive Medicine).