In vitro fertilization outcomes after intracytoplasmic sperm injection with fresh or frozen-thawed testicular spermatozoa

Objective: To compare the outcomes of intracytoplasmic sperm injection (ICS I) and in vitro fertilization (IVF) with fresh and cryopreserved testicular spermatozoa in patients with obstructive and nonobstructive azoospermia. Design: Retrospective analysis of consecutive ICSI cycles. Setting: Large urban reproductive medicine program. Patient(s): Twenty-nine patients with obstructive and nonobstructive azoosp ermia undergoing testicular sperm extraction for a total of 46 IVF-ICSI cyc les (12 fresh, 34 frozen). Intervention(s): Testicular sperm extraction, cryopreservation, and IVF-ICS I with fresh or frozen-thawed spermatozoa. Main Outcome Measure(s): Fertilization rates, embryo cleavage rates, embryo implantation rates, clinical pregnancy rates per cycle and per embryo tran sfer, and delivery and spontaneous abortion rates. Result(s): No statistically significant differences were noted in any of th e parameters examined between IVF-ICSI cycles from fresh or frozen-thawed t esticular spermatozoa. Fertilization rates were 56% with fresh vs. 61% with frozen-thawed testicular sperm, cleavage rates 92% vs. 95%, implantation r ates 26% vs. 17%, clinical pregnancy rates per cycle 33% vs; 41%, and pregn ancy rates per embryo transfer 33% vs. 45%, respectively. Delivery rates we re 75% with fresh vs. 69.2% with frozen-thawed testicular sperm, and sponta neous abortion rates 25% and 30.8%, respectively. Conclusion(s): No differences were found in IVF-ICSI outcomes between cryop reserved and fresh testicular sperm. In addition, cryopreservation provides several advantages for the patients and reproductive team. (Fertil Steril( R) 2000;73:955-60. (C) 2000 by American Society for Reproductive Medicine).