TUP1, CPH1 and EFG1 make independent contributions to filamentation in Candida albicans

The common fungal pathogen, Candida albicans, can grow either as single cel ls or as filaments (hyphae), depending on environmental conditions. Several transcriptional regulators have been identified as having key roles in con trolling filamentous growth, including the products of the TUP1, CPH1, and EFG1 genes. We show, through a set of single, double, and triple mutants, t hat these genes act in an additive fashion to Control filamentous growth, s uggesting that each gene represents a separate pathway of control. We also show that environmentally induced filamentous growth can occur even in the absence of all three of these genes, providing evidence for a fourth regula tory pathway. Expression of a collection of structural genes associated wit h filamentous growth, including HYR1, ECE1, HWP1, ALS1, and CHS2, was monit ored in strains lacking each combination of TUP1, EFG1, and CPH1. Different patterns of expression were observed among these target genes, supporting the hypothesis that these three regulatory proteins engage in a network of individual connections to downstream genes and arguing against a model wher eby the target genes are regulated through a central filamentous growth pat hway. The results suggest the existence of several distinct types of filame ntous forms of C. albicans, each dependent on a particular set of environme ntal conditions and each expressing a unique set of surface proteins.