Mz. Haider et al., High frequency of HLA-DQB1 non-Asp(57) alleles in Kuwaiti children with insulin-dependent diabetes mellitus, HUMAN HERED, 50(4), 2000, pp. 242-246
The prevalence of polymorphic amino acids at position 57 of the HLA DOB1 in
Kuwaiti children with insulin-dependent diabetes mellitus (IDDM) and nondi
abetic controls has been determined using a polymerase chain reaction-seque
nce-specific primers (PCR-SSP) method. Using this approach, 34/55 (62%) IDD
M children were found to be homozygous Ala/Ala and 19/55 (35%) were heteroz
ygous with various combinations. Amongst the IDDM children with heterozygou
s genotype at codon 57 of HLA DQB1, 6/55 (11%) had Asp/Ala, 8/55 (15%) had
Ala/Val, 4/55 (7%) had Ala/Ser and 1/55 had Asp/Val allelic combinations. W
hen considered collectively, the non-aspartate (NA) alleles were represente
d in 87% of the IDDM cases and only 13% cases had Asp(57) allele in differe
nt heterozygous combinations, while none of the IDDM subjects had a homozyg
ous Asp genotype. In nondiabetic controls, homozygous non-Asp (NA) alleles
were represented in 44% subjects, 37% of the controls were heterozygous (NA
/A) and 19% had a homozygous (A/A) genotype. These differences between the
IDDM group and the control group were found to be statistically significant
. Our data report one of the highest frequency of NA/NA residues at this lo
cus compared with that from different world populations (Sardinians, Norweg
ians, US Caucasians, US Blacks and Chinese). Copyright (C) 2000 S. Karger A
G, Basel.