HIGH-RESOLUTION LIQUID-CHROMATOGRAPHIC METHOD USING ULTRAVIOLET DETECTION FOR DETERMINATION OF ONDANSETRON IN HUMAN PLASMA

This paper describes a simple technique for extraction and a sensitive high-performance liquid chromatographic method for separation and qua ntitation of ondansetron in human plasma. The procedure involved liqui d-liquid extraction of ondansetron from plasma, reversed-phase HPLC se paration and ultraviolet detection at 305 nm. The internal standard me thod was applied for quantitation. The recovery of ondansetron was >85 %. Linearity was good throughout the concentration range anticipated i n human plasma from investigations in panic disorder (0.5-15 ng/ml, r( 2) ranging from 0.9953 to 0.9988). This method was applied to the dete rmination of plasma concentrations of ondansetron in humans.