SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR AMINOGLYCOSIDES IN BIOLOGICAL MATRICES ENABLES THE DIRECT ESTIMATION OF BACTERIAL DRUG UPTAKE

Authors
STEAD DA RICHARDS RME
Citation
Da. Stead et Rme. Richards, SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR AMINOGLYCOSIDES IN BIOLOGICAL MATRICES ENABLES THE DIRECT ESTIMATION OF BACTERIAL DRUG UPTAKE, Journal of chromatography B. Biomedical sciences and applications, 693(2), 1997, pp. 415-421
Citations number
13
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
693
Issue
2
Year of publication
1997
Pages
415 - 421
Database
ISI
SICI code
0378-4347(1997)693:2<415:SHLAFA>2.0.ZU;2-M
Abstract
Following the development of a sensitive high-performance liquid chrom atographic (HPLC) assay for gentamicin in biological matrices, the uti lity of this assay for the determination of other clinically important aminoglycosides (neomycin, netilmicin and sisomicin) in bacterial cul ture media or plasma is demonstrated. The high sensitivity of the assa y enables direct measurement of the aminoglycoside content of bacteria l cells cultured in the presence of unlabelled drug.