DETERMINATION OF THE ANTICANCER AGENT CI-980 IN PLASMA BY ACHIRAL LIQUID-CHROMATOGRAPHY ON A PIRKLE-TYPE STATIONARY-PHASE

A high-performance liquid chromatographic assay has been developed and validated for the determination of the anticancer agent CI-980 in pla sma samples from pediatric patients. After ether er,traction from alka line plasma, the CI-980 was chromatographed on a (R)-N-(3,5-dinitroben zoyl)phenylglycine stationary phase using a mobile phase composed of h exane-isopropanol (70:30, v/v) modified with 1% acetonitrile. The meth od was linear over a 0.25 to 25.00 ng/ml range and the intra- and inte r-day coefficients of variation (C.V.s) were less than 15%. The method was applied to the determination of the plasma concentration-time pro file for a pediatric patient receiving 3.5 mg/m(2) of CI-980 per day a s a continuous 72-h infusion.