P. Husek et P. Matucha, SIMPLE APPROACH FOR ANALYSIS OF PLASMA OXO-ACIDS, HYDROXY-ACIDS AND DICARBOXYLIC-ACIDS, Journal of chromatography B. Biomedical sciences and applications, 693(2), 1997, pp. 499-502
Citations number
12
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Following deproteinization of plasma with organic solvents the superna
tant was shaken with hexane and cation-exchange resin in an Eppendorf
tube to remove fatty and amino acids and the medium was subjected to d
irect treatment with ethyl chloroformate under catalytic influence of
pyridine. A subsequent extraction of the immediately formed ethyl este
rs with a drop of chloroform enabled us to subject the sample to gas c
hromatographic (GC) analysis. Since ketocarboxylic acids do not requir
e a preliminary oximation the total time of sample workup and analysis
takes only several minutes.