Humoral immune response was studied in broilers against inclusion body hepa
titis (IBH) inactivated oil adjuvanted vacccine, and virulent PDRC-1 strain
of the IBH virus by using agar gel immunodiffusion (AGID) and enzyme linke
d immunosorbent assay (ELISA). IBH antibody was detected from seventh onwar
ds in IBH vaccinated and infected groups of chickens by both the rests. Inf
ected and challenged groups exhibited strong immune response when compared
with the vaccinated group. The immunosuppressed group of chickens infected
with IBH virus showed poor development of IBH antibody.