The anti-bacterial action of diclofenac shown by inhibition of DNA synthesis

Most strains of Gram-positive and Gram-negative bacteria were inhibited by 50-100 mg/l of the anti-inflammatory agent, diclofenac sodium (Dc). In vivo test using 30 or 50 mu g Dc per 20 g mouse (Swiss Albino variety) signific antly (P < 0.001) protected the animals when challenged with 50 MLD of a vi rulent Salmonella typhimurium. The anti-bacterial action of Dc was found to be due to inhibition of DNA synthesis which was demonstrated using 2 mu Ci (H-3) deoxythymidine uptake. (C) 2000 Elsevier Science B.V. and Internatio nal Society of Chemotherapy. All rights reserved.