Illegitimate villin transcripts in normal bone marrow precludes detection of colon cancer micrometastases

Authors
Salbe, C De Cremoux, P Bonneton, C Manet, S Almeida, A Magdelenat, H Bourstyn, E Robine, S
Citation
C. Salbe et al., Illegitimate villin transcripts in normal bone marrow precludes detection of colon cancer micrometastases, INT J B MAR, 15(1), 2000, pp. 41-43
Citations number
10
Categorie Soggetti
Oncology
Journal title
INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS
ISSN journal
03936155 → ACNP
Volume
15
Issue
1
Year of publication
2000
Pages
41 - 43
Database
ISI
SICI code
0393-6155(200001/03)15:1<41:IVTINB>2.0.ZU;2-4
Abstract
Villin is a specific marker for normal and tumoral colon tissue. We have de veloped a highly sensitive assay using reverse transcription (RT) and real- time PCR to detect villin transcripts. The sensitivity of detection is one colon cancer cell. However high levels of illegitimate villin transcripts w ere observed in normal bone marrow precluding the use of villin RT-PCR for routine detection of colon cancer cells in bone marrow of patients with col on cancer.