Evaluation of the PASCO strep plus broth microdilution antimicrobial susceptibility panels for testing Streptococcus pneumoniae and other streptococcal species

Antimicrobial resistance continues to increase worldwide among isolates of Streptococcus pneumoniae and other species of streptococci, increasing rate s of penicillin resistance, particularly in viridans group streptococci, an d resistance to multiple classes of antimicrobial agents, including beta-la ctams, macrolides, and fluoroquinolones, in pneumococci have increased the importance of having accurate antimicrobial susceptibility testing results for guiding therapy. One commercial method of assessing resistance in strep tococci is the PASCO Strep Plus panel. This broth microdilution-based metho d has recently been expanded to include a variety of newer antimicrobial ag ents. Therefore, we compared the results of the new PASCO Strep Plus panels for 26 antimicrobial agents against the results generated using the Nation al Committee for Clinical Laboratory Standards (NCCLS) broth microdilution reference method for 75 pneumococci and 68 other streptococcal isolates. On ly 4 (0.2%) very major errors (all with pneumococci and each with a differe nt antimicrobial agent) were observed. There were 5 (0.3%) major errors obs erved with pneumococci (each with a different antimicrobial agent), but onl y 1 major error with nonpneumococcal streptococci. All of the very major an d major errors resolved on retesting. Of the 65 (3.9%) and 17 (1.6%) minor errors observed with pneumococci and other streptococci, respectively, all were within 1 dilution of the broth microdilution reference MIC result. Thu s, the PASCO Strep Plus panel has comparable accuracy to the NCCLS broth mi crodilution reference method.