Multiplex PCRs for identification of Escherichia coli virulence genes

Authors
Pass, MA Odedra, R Batt, RM
Citation
Ma. Pass et al., Multiplex PCRs for identification of Escherichia coli virulence genes, J CLIN MICR, 38(5), 2000, pp. 2001-2004
Citations number
15
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
38
Issue
5
Year of publication
2000
Pages
2001 - 2004
Database
ISI
SICI code
0095-1137(200005)38:5<2001:MPFIOE>2.0.ZU;2-7
Abstract
PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Spe cificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.