K. Podual et al., Glucose-sensitivity of glucose oxidase-containing cationic copolymer hydrogels having poly(ethylene glycol) grafts, J CONTR REL, 67(1), 2000, pp. 9-17
Glucose oxidase and catalase were immobilized on poly(diethylaminoethyl met
hacrylate-g-ethylene glycol) gels by copolymerization of the constituent mo
nomers and the functionalized enzyme solutions. The hydrogels were prepared
in the form of discs and microparticles. The amount and the activity of en
zymes immobilized in the matrix were determined. The hydrogels were tested
for their response to glucose by exposing microparticles to varying concent
rations of glucose. The generation of gluconic acid as a result of the reac
tion of glucose with oxygen was investigated as a function of polymer param
eters, such as crosslinking ratio and enzyme loading. Pulsatile variation o
f the glucose concentration was used to confirm the glucose-dependent swell
ing properties of these hydrogels. (C) 2000 Elsevier Science B.V. All right
s reserved.