AN OCTAMER ELEMENT FUNCTIONS AS A REGULATORY ELEMENT IN THE DIFFERENTIATION-RESPONSIVE CD11C INTEGRIN GENE PROMOTER - OCT-2 INDUCIBILITY DURING MYELOMONOCYTIC DIFFERENTIATION

Citation
C. Lopezrodriguez et al., AN OCTAMER ELEMENT FUNCTIONS AS A REGULATORY ELEMENT IN THE DIFFERENTIATION-RESPONSIVE CD11C INTEGRIN GENE PROMOTER - OCT-2 INDUCIBILITY DURING MYELOMONOCYTIC DIFFERENTIATION, The Journal of immunology, 158(12), 1997, pp. 5833-5840
Citations number
47
Categorie Soggetti
Immunology
Journal title
The Journal of immunology
ISSN journal
00221767 → ACNP
Volume
158
Issue
12
Year of publication
1997
Pages
5833 - 5840
Database
ISI
SICI code
0022-1767(1997)158:12<5833:AOEFAA>2.0.ZU;2-B
Abstract
The integrin CD11c/CD18 mediates leukocyte adhesion to endothelium and other cell types and is a receptor for LPS, iC3b, and fibrinogen, CD1 1c expression is restricted to myeloid and activated B cells, is regul ated during leukocyte differentiation, and constitutes a diagnostic to ol for hairy cell leukemia. Mapping of in vivo DNA-protein interaction s in the CD11c proximal promoter revealed three adjacent myeloid-speci fic interactions, one of which lies on an octamer consensus sequence, ATTT GCAT (Oct185), Oct185 disruption increased the CD11c promoter act ivity while decreasing its myeloid differentiation responsiveness, ind icating that Oct185 contributes to the activity of the CD11c promoter and suggesting that Oct185 is a negative regulatory element whose func tion changes during myeloid differentiation, Oct185 is recognized by t he ubiquitous Oct-1 factor in all cell lineages and by Oct-2 in B lymp hoid lineage cells, Unexpectedly, Oct-2 binding to Oct185 was induced de novo upon monocytic differentiation of U937 and HL-60 cells but not during HL-60 granulocytic differentiation, as determined by electroph oretic mobility shift assays and immunochemical studies, and Oct-2 com plexes were also observed in cultured adherent monocytes, Western blot ting showed that the pattern of Oct-2 isoforms in myeloid cells is sim ilar to that seen in B cells, The Oct-2 up-regulated expression in dif ferentiating myeloid cells and its binding to the Oct185 negative regu latory element suggests its involvement in the differentiation-regulat ed activity of the CD11c promoter and might represent an important par ameter for the myeloid- and B cell-restricted expression of the CD11c/ CD18 integrin and other molecules with similar patterns of expression.