M. Boda et al., The caffeine metabolic ratio as an index of xanthine oxidase activity in clinically active and silent celiac patients, J PED GASTR, 29(5), 1999, pp. 546-550
Citations number
28
Categorie Soggetti
Pediatrics,"Medical Research General Topics
Journal title
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
Background: The xanthine oxidoreductase system has been identified as one o
f the main sources of free radicals responsible for various forms of tissue
injury. Because the intestinal villi are an important location of this enz
yme, it was of interest to study the role of xanthine oxidase in gluten-sen
sitive celiac enteropathy, associated with characteristic villous atrophy.
Measured by a noninvasive method, the ratio of caffeine metabolites excrete
d in the urine after a caffeine challenge had previously been shown to be i
ndicative of the total xanthine oxidase activity of the patient.
Methods: The study involved 22 children with gluten-challenged celiac disea
se, exhibiting subtotal villous atrophy in specimens from the third intesti
nal biopsy in accordance with ESPGHAN criteria. Ten of the patients display
ed overt clinical symptoms (active form), whereas 12 had no symptoms (silen
t form). Urinary caffeine metabolites were determined by high-pressure liqu
id chromatography. The total in vivo xanthine oxidase activity was expresse
d as the caffeine metabolite index.
Results: In patients with active celiac disease the xanthine oxidase activi
ty index was considerably higher, whereas in those with silent disease it w
as significantly lower than the control value. A significant negative corre
lation was shown between the index indicative of xanthine oxidase activity
and the serum iron level of the patients.
Conclusions: Activation of xanthine oxidase may play a role in the pathogen
esis of active celiac disease with definite malabsorption, gastrointestinal
symptoms, and anemia. The caffeine test reflects the difference in the pat
hogenetic mechanism leading to the mucosal lesion and clinical symptoms of
active and silent forms of celiac disease.